Micrornas as molecular tools modulating muscle differentiation and regeneration
- Amelia Aranega Jiménez Director
- Francisco Hernandez Torres Co-director
Defence university: Universidad de Jaén
Fecha de defensa: 17 February 2023
- Juan Manuel Fernández Costa Chair
- María Luisa del Moral Leal Secretary
- Andrea Münsterberg Committee member
Type: Thesis
Abstract
Satellite cells (SCs), muscle stem cells, display functional heterogeneity and dramatic changes linked to their regenerative capabilities are associated with muscle-wasting diseases, such as muscular dystrophies or ageing. SC behavior is related to endogenous expression of the myogenic transcription factor Myf5 and the propensity to cell proliferation. In this doctoral Thesis we reported a role for microRNA-106b-5p reinforcing Myf5 inhibition and blocking cell proliferation in a subset of highly quiescent SC population. We showed that microRNA-106b-5p down-regulation in SCs was required proper muscle repair. Additionally, miR-106b inhibition promotes muscle regeneration enhancing the regenerative capabilities of dystrophic muscles and aged SCs, proposing antimiR-106b-5p treatment as a new therapeutic approach for muscle-wasting diseases. Finally, we observed that miR-106b-5p defines a subset of myogenic progenitor cell population during embryonic myogenesis and fetal myogenesis, suggesting this subpopulation will give rise different adult SC populations.