Sensibilidad a cobre y cinc y corresistencias en bacterias de alimentos vegetales no procesados

  1. CIDRE FERNÁNDEZ, ISMAEL
Supervised by:
  1. Rubén Pérez Pulido Director
  2. Antonio Gálvez del Postigo Ruiz Co-director

Defence university: Universidad de Jaén

Fecha de defensa: 24 July 2017

Committee:
  1. Manuel Martínez Bueno Chair
  2. Elena Ortega Morente Secretary
  3. Samir Ananou Committee member
Department:
  1. CIENCIAS DE LA SALUD

Type: Thesis

Teseo: 555174 DIALNET

Abstract

Copper and zinc resistance was studied in 506 isolates (aerobic mesophiles) from 12 different fresh produce products. Over 50% of isolates had minimum inhibitory concentrations (MICs) for copper sulfate above 16 mM, and over 40% had MICs > 4 mM for zinc chloride. 51 isolates with high MICs for both copper and zinc were identified by 16S rDNA sequencing. Forty of them were gram-negative bacteria belonging to genera Pseudomonas (28), Enterobacter (7), Serratia (4) and Leclercia (1). A high incidence of genes from the pco multicopper oxidase cluster and the sil cluster involved in copper and silver resistance, as well as the chromate resistance gene chrB. Remarkably, a high percentage of the studied isolates carried both pco and sil genetic determinants. Results suggest that copper and zinc resistance as well as metal resistance genes are widespread in bacteria from fresh produce.