Nuevos materiales biomiméticos y biotintas con aplicación en ingeniería regenerativa de cartílago y piel

Abstract

Regenerative engineering (RE) is an interdisciplinary amalgam of technological fields that combine tissue engineering, material science, stem cell biology, developmental biology, and clinical translation to manufacture complex artificial tissues. Tissue engineering (TE) has been described as the emerging fields of knowledge whose objective is to create artificial tissues and/or organs designed for mimicking their native form. Regenerative engineering combines cells, biomaterials, and biologically active molecules with proper manufacturing platforms to produce complex tissues. The main objective is to create functional constructs that can replace, preserve, or improve damaged tissues or organs. Artificial cartilage and skin substitutes are examples of devices that have been approved by the Food and Drug Administration (FDA), however, their use in humans is currently limited. Although TE manufacture strategies led to the first generations of engineered tissues, such processes usually consume time and are restricted to having mainly flat and pre-determined geometries. Furthermore, they also exhibit high manufacture costs, as well as human factors regarding surgeons and patients that may influence the success of the implantation. Summarizing, this doctoral thesis offers robust and extensive studies in which b- TPUe was validated for 3D bioprinting applications. Also, the design and biofabrication of a BT skin substitute are presented, demonstrating its biological and mechanical properties in vitro and in vivo, encouraging its future clinical application in RE of skin injuries.