Estudio sobre la influencia de diferentes dietas altas en grasa en enterococos intestinales murinos
- SÁNCHEZ ARCAS, BEATRIZ
- Magdalena Martínez Cañamero Directora
- Antonio Gálvez del Postigo Ruiz Codirector
- Antonio Cobo Molinos Codirector/a
Universidad de defensa: Universidad de Jaén
Fecha de defensa: 12 de marzo de 2020
- Mª Carmen Millán Pérez Presidente/a
- Maria del Rosario Lucas Lopez Secretario/a
- María Belén Fernández Luque Vocal
Tipo: Tesis
Resumen
In this work, a comparative study on potential risks was carried out in a collection of 50 enterococci isolated from faeces of mice fed a standard diet or enriched in different fats: extra virgin olive oil, refined olive oil or butter, at different time points. The strains were evaluated biochemically and genetically characterized. The biochemical study revealed that there are statistically significant differences between the different diets and times in each of the assays. The most representative species was E. faecalis and the genetic study for its taxonomic characterization revealed the presence of essentially two sequence types MLST, ST9 in the standard diet isolates and ST40 in the butter-enriched diet. We conclude with a proteomic study where we observe the differences in the protein fractions of the two STs and its possible relationship with their different invasive ability.