Effect of solar and artificial UV radiation on photosynthetic performance and carbonic anhydrase activity in intertidal macroalgae from southern Spain

  1. B Viñegla 1
  2. F López-Figueroa 2
  1. 1 Department of Plant Biology, Animal Biology and Ecology, University of Jaén, Jaén, Spain.
  2. 2 Department of Ecology and Geology, University of Málaga, Málaga, Spain.
Revista:
Ciencias marinas

ISSN: 0185-3880,  2395-9053

Any de publicació: 2009

Volum: 35

Número: 1

Pàgines: 59-74

Tipus: Article

DOI: 10.7773/CM.V35I1.1512 DIALNET GOOGLE SCHOLAR lock_openAccés obert editor

Altres publicacions en: Ciencias marinas

Objectius de Desenvolupament Sostenible

Resum

The effect of UV radiation on photosynthetic rate and carbon uptake, estimated as carbonic anhydrase activity, was studied by incubating intertidal algae, Fucus spiralis (Phaeophyta) and Ulva olivascens (Chlorophyta), under both artificial and solar radiation. In these experiments, treatments excluding UV-B or UV-A + UV-B radiations were applied. Thalli of U. olivascens and F. spiralis showed completely different responses when exposed to artificial radiation and three different light treatments (PAR + UV-A + UV-B, PAR + UV-A, and only PAR). After exposure for 24 h, photosynthetic activity increased in F. spiralis, although the presence of UV-B slightly reduced the increase, whereas U. olivascens showed an inverse pattern, with decreasing photosynthetic rates in all the treatments and with the lowest decrease in the presence of UV-B radiation. Surprisingly, the presence of UV-B stimulated carbon uptake, though this effect was more clear in the case of F spiralis, while a similar inhibitory effect of UV-A radiation was found in both species. Thalli of U. olivascens and F. spiralis were also exposed to solar radiation for daily and yearly periods. The photosynthetic rate was coupled to incident irradiance in F. spiralis, whereas in U. olivascens a significant decrease in the presence of both UV-A or UV-A + UV-B was found. A significant increase of carbon uptake in the presence of UV-B radiation was observed in U. olivascens, while no clear pattern was found in F. spiralis under any of the light treatments. During an annual cycle, carbon uptake in U. olivascens decreased in winter, compared with the initial levels, in the PAR and PAR + UV-A treatments. The high level of carbon uptake was maintained until the end of spring, when higher levels of incident energy might promote photoinhibition (in the presence of UV-B). The absence of a general response to UV radiation in the macroalgae studied may be due to their different photoprotection mechanisms, physiological-morphological characteristics, and acclimation to environmental conditions.

Referències bibliogràfiques

  • Abdala-Díaz, RT,Cabello-Pasini, A,Pérez-Rodríguez, E,Conde-Alvarez, RM,Figueroa, F. (2006). Daily and seasonal variations of optimum quantum yield and phenolic compounds in Cystoseira tamariscifolia (Phaeophyta).. Mar. Biol. 148. 459-465
  • Altamirano, M,Flores-Moya, A,Figueroa, F. (2000). Long-term effect of natural sunlight under various ultraviolet radiation conditions on growth and photosynthesis of intertidal Ulva rigida (Chlorophyceae) cultivated in situ. Bot. Mar. 43. 119-126
  • Altamirano, M,Flores-Moya, A,Conde, F,Figueroa, F. (2000). Growth seasonality, photosynthetic pigments and C and N content in relation to environmental factors: A field study on Ulva olivascens (Ulvales, Chlorophyta). Phycologia. 39. 50-58
  • Altamirano, M,Flores-Moya, A,Figueroa, F. (2003). Effects of UV radiation and temperature on growth germlings of three species of Fucus (Phaeophyceae). Aquat. Bot. 75. 9-20
  • Bañares, E,Altamirano, M,Figueroa, FL,Flores-Moya, . (2002). Influence of UV radiation on growth of sporelings of three non-geliculate coralline red algae from southern Iberian Peninsula. Phycol. Res. 50. 23-30
  • Bischof, K,Hanelt, D,Wienke, . (1998). UV-radiation can affect depth-zonation of Antarctic macroalgae. Mar. Biol. 131. 597-605
  • Bischof, K,Peralta, G,Krabs, G,van de Poll, WH,Pérez-Llorens, JL,Breeman, A. (2002). Effects of solar UV-B radiation on canopy structure of Ulva communities from southern Spain. J. Exp. Bot.. 53. 2411-2421
  • Bischof, K,Gómez, I,Molis, M,Hanelt, D,Karsten, U,Lüder, U,Roleda, MY,Zacher, K,Wiencke, . (2006). Ultraviolet radiation shapes seaweed communities. Rev. Environ. Sci. Biotechnol. 5. 141-166
  • Braune, W,Döhler, . (1996). Impact of UV-B radiation on 15N-ammonium and 15N-nitrate uptake by Haematococcus lacustris (Volvocales): II. The influence of a recovery period. J. Plant Physiol. 149. 349-357
  • Büchel, C,Wilhelm, . (1993). In vivo analysis of slow chlorophyll induction kinetics in algae: Progress, problems and perspectives. Photochem. Photobiol. 58. 137-148
  • Cabello-Pasini, A,Figueroa, F. (2005). Effect of nitrate concentration on the relation between photosynthetic oxygen evolution and electron transport rate in Ulva rigida (Chlorophyta). J. Phycol. 41. 1169-1177
  • Döhler, G,Hagmeier, E,David, C. (1995). Effects of solar and artificial UV irradiation on pigments and assimilation of 15N ammonium and 15N nitrate by macroalgae. J. Photochem. Photobiol. B: Biol. 30. 179-187
  • Dring, MJ,Wagner, A,Boeskov, J,Lüning, . (1996). Sensitivity of intertidal and subtidal red algae to UVA and UVB radiation, as monitored by chlorophyll fluorescence measurements: Influence of collection depth and season and length of irradiation. Eur. J. Phycol. 31. 293-302
  • Figueroa, FL,Viñegla, . (2001). Effects of solar UV radiation on photosynthesis and enzyme activities (carbonic anhydrase and nitrate reductase) in marine macroalgae from southern Spain. Rev. Chil. Hist. Nat. 74. 237-249
  • Figueroa, FL,Salles, S,Aguilera, J,Jiménez, C,Mercado, J,Viñegla, B,Flores-Moya, A,Altamirano, . (1997). Effects of solar radiation on photoinhibition and pigmentation in the red alga Porphyra leucosticta. Mar. Ecol. Prog. Ser.. 151. 81-90
  • Figueroa, FL,Jiménez, C,Viñegla, B,Pérez-Rodríguez, E,Aguilera, J,Flores-Moya, A,Altamirano, M,Lebert, M,Häder, D. (2002). Effects of solar UV radiation on photosynthesis of the marine angiosperm Posidonia oceanica from southern Spain. Mar. Ecol. Prog. Ser. 230. 59-70
  • Figueroa, FL,Conde-Alvarez, R,Gómez, . (2003). Relations between electron transport rates determined by pulse amplitude modulated fluorescence and oxygen evolution in macroalgae under different light conditions. Photosynthesis Res.. 75. 259-275
  • Figueroa, FL,Nygard, C,Ekelund, N,Gómez, . (2003). Photobiological characteristics and photosynthetic UV responses in two Ulva species (Chlorophyta) from southern Spain. J. Photochem. Photobiol. 72. 35-44
  • Flores, E,Herrero, . (1994). The Molecular Biology of Cyanobacteria. Kluwer Academic Publ. Dordrecht.
  • Flores-Moya, A,Gómez, I,Viñegla, B,Altamirano, M,Pérez-Rodríguez, E,Maestre, C,Caballero, RM,Figueroa, F. (1998). Effects of solar radiation on the endemic Mediterranean red alga Rissoella verruculosa: Photosynthetic performance, pigment content and the activities of enzymes related to nutrient uptake. New Phytol. 139. 673-683
  • Flores-Moya, A,Hanelt, D,Figueroa, FL,Altamirano, M,Viñegla, B,Salles, . (1999). Involvement of solar UV-B radiation in recovery of inhibited photosynthesis in the brown alga Dictyota dichotoma (Hudson) Lamouroux. J. Photochem. Photobiol. 49. 129-135
  • Frederick, JE,Soulen, PF,Diaz, SB,Smolskaia, I,Booth, CR,Lucas, T,Neuschuler, . (1993). Solar ultraviolet irradiance observed from South Argentina: September 1999 to March 1991. J Geophys. Res. 98. 88-91
  • Gao, Y,Smith, GJ,Alberte, R. (1992). Light regulation of nitrate reductase in Ulva fenestrata (Chlorophyceae): I. Influence of light regimes on nitrate reductase activity. Mar. Biol. 112. 691-696
  • Gerber, S,Häder, D. (1993). Effects of solar radiation on motility and pigmentation of 3 species of phytoplancton. Env. Exp. Bot.. 33. 515-521
  • Gómez, I,Pérez-Rodríguez, E,Viñegla, B,Figueroa, FL,Karsten, . (1998). Effects of solar radiation on photosynthesis, UV-absorbing compounds and enzyme activities of the green alga Dasycladus vermicularis from southern Spain. J. Photochem. Photobiol. B: Biol. 47. 46-57
  • Gómez, I,Figueroa, F,Ulloa, N,Morales, V,Lovengreen, C,Huovinen, C,Hess, . (2004). Patterns of photosynthesis in 18 species of intertidal macroalgae from southern Chile. Mar. Ecol. Prog. Ser. 270. 103-116
  • Gómez, I,Ulloa, N,Oréstegui, . (2005). Morpho-functional patterns of photosynthesis and UV sensitivity in the kelp Lessonia nigresscens (Laminaria, Phaeophyta). Mar. Biol.. 148. 231-240
  • Gordillo, FJL,Segovia, M,Figueroa, F. (2004). Cyclic AMP levels in several macroalgae and their relation to light quantity and quality. J. Plant Physiol. 161. 211-217
  • Häder, DP,Figueroa, F. (1997). Photoecophysiology of marine macroalgae. Photochem. Photobiol. 66. 1-14
  • Häder, DP,Lebert, M,Mercado, J,Aguilera, J,Salles, S,Flores-Moya, A,Jiménez, C,Figueroa, F. (1996). Photosynthetic oxygen production and PAM fluorescence in the brown alga Padina pavonica measured in the field under solar radiation. J. Phycol. 32. 614-620
  • Haglund, K,Björk, M,Ramazanov, Z,García-Reina, G,Pedersén, . (1992). Role of carbonic anhydrase in photosynthesis and inorganic-carbon assimilation in the red alga Gracilaria tenuistipitata. Planta. 187. 275-281
  • Han, Y-S,Han, . (2005). UV-B induction of UV-B protection in Ulva pertusa (Chlorophyta). J. Phycol. 41. 523-530
  • Hanelt, . (1998). Capability of dynamic photoinhibition in Arctic macroalgae is related to their depth distribution. Mar. Biol. 131. 361-369
  • Henry, BE,van Alstyne, K. (2004). Effects of UV radiation on growth and phlorotannins in Fucus gardneri (Phaeophyceae) juveniles and embryos. J. Phycol. 40. 527-533
  • Jassby, AD,Platt, . (1976). Mathematical formulation of the relationship between photosynthesis and light for phytoplankton. Limnol. Oceanogr. 21. 540-547
  • Jiménez, C,Figueroa, FL,Salles, S,Aguilera, J,Mercado, J,Viñegla, B,Flores-Moya, A,Lebert, M,Häder, D. (1998). Effects of solar radiation on photosynthesis and photoinhibition in red macrophytes from an intertidal system of southern Spain. Bot. Mar. 41. 329-338
  • Karsten, U,Bischof, K,Hanelt, D,Tüg, H,Wiencke, . (1999). The effect of UV radiation on photosynthesis and UV screen substances in the endemic Arctic macroalga Devalarea ramentacea (Rhodophyta). Physiol. Plant. 105. 58-66
  • Kirk, JT. (1976). Light and Photosynthesis in the Aquatic Ecosystems. Cambridge Univ. Press. Cambridge.
  • Korbee, N,Figueroa, FL,Aguilera, . (2005). Effect of light quality on the accumulation of photosynthetic pigments, proteins and mycosporine-like amino acids in the red alga Porphyra leucosticta (Bangiales, Rhodophyta). J. Photochem. Photobiol. 80. 71-78
  • Krause, GH,Weis, . (1991). Chlorophyll fluorescence and photosynthesis: The basics. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42. 313-349
  • Kumar, A,Sinha, RP,Häder, D. (1996). Effect of UV-B on enzymes of nitrogen metabolism in the cyanobacterium Nostoc calcicola. J. Plant Physiol. 148. 86-91
  • Lopes, PF,Oliveira, MC,Colepicolo, . (1997). Diurnal fluctuation of nitrate reductase activity in the marine red alga Gracilaria tenuistipitata (Rhodophyta). J. Phycol. 33. 225-231
  • Maegawa, M,Kunieda, M,Kida, . (1993). The influence of ultraviolet radiation on the photosynthetic activity in several red algae from different depths. Jap. J. Phycol. 41. 207-214
  • Mercado, JM,Gordillo, FJ,Figueroa, FL,Niell, F. (1998). External carbonic anhydrase and affinity to inorganic carbon in intertidal macroalgae. J. Exp. Mar. Biol. Ecol. 221. 209-220
  • Mori, T,Binder, B,Johnson, C. (1996). Circadian gating of cell division in cyanobacteria growing with average doubling times of less than 24 hours. Proc. Nat. Acad. Sci. USA. 93. 10183-10188
  • Quiñones, MA,Aparicio, P. (1990). Flavin type action spectrum of nitrate utilization by Monoraphidium brauni. Photochem. Photobiol.. 51. 689-692
  • Pavia, H,Cervin, G,Lindgren, A,Aberg, P. (1997). Effects of UV-B radiation and simulated hebivory on phlorotannins in the brown alga Ascophyllum nodosum. Mar. Ecol. Prog. Ser.. 157. 139-146
  • Roleda, MY,Wienke, C,Hanelt, . (2006). Thallus morphology and optical characteristics affect growth and DNA damage by UV radiation in juvenile Arctic Laminaria sporophytes. Planta. 223. 407-417
  • Rüdiger, W,López-Figueroa, . (1992). Yearly review: Photoreceptors in algae. Photochem. Photobiol. 55. 949-954
  • Schreiber, U,Endo, T,Mi, H,Asada, . (1995). Quenching analysis of chlorophyll fluorescence by saturation pulse method: Particular aspects relating to the study of eukaryotic algae and cyanobacteria. Plant Cell Physiol. 36. 873-882
  • Sokal, PR,Rohlf, F. (1995). Biometry. WH Freeman ed. San Francisco.
  • Van de Poll, WH,Eggert, A,Buma, AGJ,Breeman, A. (2001). Effects of UV-B induced DNA damage and photoinhibition of growth of temperate marine red macrophytes: Habitat-related differences in UV-B tolerance. J. Phycol. 37. 30-37
  • Villafañe, V,Sunfbãck, C,Figueroa, FL,Helbling, . (2003). UV Effects in Aquatic Organisms and Ecosystems. Comprehensive Series in Photosciences.
  • Viñegla, . (2000). Efecto de la radiación ultravioleta sobre las actividades enzimáticas relacionadas con el metabolismo del carbono y el nitrógeno en microalgas y fanerógamas marinas. 302
  • Viñegla, B,Segovia, M,Figueroa, F. (2006). Effect of artificial UV radiation on carbon and nitrogen metabolism in the macroalgae Fucus spiralis L. and Ulva olivascens Dangeard. Hydrobiologia. 560. 31
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