Glutamil aminopeptidasa en orina, en microvesículas y en exosomas como marcador de la nefrotoxicidad inducida por el cisplatino en ratas

Resumo

The main objective of this Doctoral Thesis is to investigate whether the excretion of glutamyl aminopeptidase (GluAp), is a marker of cisplatin-induced nephrotoxicity in rats. In a first experiment, the early excretion of GluAp as determined by kinetic fluorimetry, ELISA and immunoblotting was found to be increased in cisplatin-treated rats and it showed a predictive value over renal dysfunction. In other work, it was demonstrated that GluAp in microvesicular and exosomal urinary fractions was also correlated with renal dysfunction if data were normalized by daily total excretion, by concentration of urinary creatinine or by total protein in each fraction. It is concluded that excretion of GluAp in urine is a marker of renal damage induced by cisplatin in rats that can be useful to evaluate renal tubular damage independently of creatinine excretion.