Análisis citogenético y molecular del ADN repetitivo en anfibios

Abstract

Amphibians are a group of animals with wide distribution and great biological importance. Despite this fact, few in-depth studies on their genome have been done. In the present work we have studied several species of the Bufonidae family from a cytogenetic and molecular point of view. The results obtained allowed to identify and characterize, from a cytogenetic and molecular point of view, 4 different repetitive DNA families have been identified and characterised from a molecular and cytogenetic point of view. In addition, different models involved in the evolution of these repeated DNA sequences have been identified. The present work shows that Bufonidae family has a large library of repetitive DNAs, with diverse variants and different repetitive DNAs that have been differentially amplified in each species or genus. These repetitive DNAs are responsible for the interspecific variation observed in the analyzed species of the family Bufonidae and can be used as a complementary tool to establish taxonomic and population relationships in this amphibian family.