Detección de sustancias dopantes en orina mediante cromatografía de líquidos/espectrometría de masas de tiempo de vuelo
- Domínguez Romero, Juan Carlos
- Antonio Molina Díaz Director
- Juan Francisco García Reyes Director
Defence university: Universidad de Jaén
Fecha de defensa: 27 February 2015
- Luis Fermín Capitán Vallvey Chair
- Carmen García Ruiz Secretary
- Miguel de la Guardia Committee member
Type: Thesis
Abstract
In the last years, liquid chromatography/mass spectrometry (LC-MS) has replaced chromatography/mass spectrometry (GC-MS) as the main technique in antidoping laboratories. LC-MS provides some advantages in ease of sample treatment compared to gas chromatography (GC), although GC methods are still in use particularly for class-specific applications. The requirements and features of sport drug testing map well against the potential of liquid chromatography high resolution mass spectrometry using time-of-flight analyzer (LC-TOFMS). The main advantage of this approach in comparison with targeted measurements based on the acquisition of fixed parent/fragment ion transitions is the comprehensive acquisition of the entire data, which provides the possibility to retrospectively scrutinize the analytical data for formerly unknown compounds or new species that eventually become relevant from the point of view of sport drug testing.